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Methods – Castro-Alamancos lab

Methods

We use these methods:

  • Electrophysiology
    • whole-cell recordings
    • single-unit recordings
    • field potentials
    • EMG
  • Neuropharmacology
  • Optogenetics
  • DREADDS
  • Calcium imaging fiber photometry
  • Microdialysis
  • Behavioral conditioning
  • Video tracking
  • Histology
    • Single-cell reconstructions
    • AAV injections and cannula tracing

… in mice that are under these conditions …

  • Freely behaving (tethered)
  • Head-fixed
  • Anesthetized
  • Brain slices (in vitro)

Rigor is an important consideration in our work. Our experiments and analyses are automated (coded), so there are fewer confounding variables.